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Assembly

Assembly bins (FASTAs)

Assembled contigs are found in the Assembly folder under Metagenomic Bins as .fna (FASTA) files. These files contain all organisms with sufficient depth in the submitted sequencing data to be assembled. Details on each bin, such as their completeness (eg. BUSCO count), antimicrobial resistance profile and more are found in the summary and per-sample reports.

A search bar under the “Assembly” tab enables you to search for assemblies across all samples in a given analysis. From there, you can click the “FNA” button beside each record to download the raw assembly file. Or, for smaller assemblies, you can click the “BLAST” button to submit the files directly to NCBI BLAST for further analysis (a popup will occur to open BLAST in a separate window).

Tip

Output FASTA files are sorted by contig length, from longest to shortest contig. Output FASTAs also have contig length and plasmid information in their sequence header.